Cross-linked collagen triple helices by oxime ligation. Molecular magnetic resonance imaging of lung fibrogenesis with an oxyamine-based probe. Oximes and hydrazones in bioconjugation: mechanism and catalysis. Hydrolytic stability of hydrazones and oximes. Cyclic peptides for efficient detection of collagen. Targeting collagen strands by photo-triggered triple-helix hybridization. Optimization of interstrand interactions enables burn detection with a collagen-mimetic peptide. Visualizing collagen proteolysis by peptide hybridization: from 3D cell culture to in vivo imaging. Collagen-based biomaterials for wound healing. Lysyl oxidase in development, aging and pathologies of the skin. Changes in collagen cross-linking and lysyl oxidase by estrogen. Identification of an elastin cross-linking domain that joins three peptide chains: possible role in nucleated assembly. Lysyl oxidases: a novel multifunctional amine oxidase family. An enantioselective fluorimetric assay for alcohol dehydrogenases using albumin-catalyzed beta-elimination of umbelliferone. Accelerating turnover frequency in nucleic acid templated reactions. Synthesis of a fluorescent analogue of paclitaxel that selectively binds microtubules and sensitively detects efflux by P-glycoprotein. Structure-guided engineering of a pacific blue fluorophore ligase for specific protein imaging in living cells. Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Strategy for specific fluorescence imaging of monoamine oxidase a in living cells. Activity-based fluorescent reporters for monoamine oxidases in living cells. Design of optical switches as metabolic indicators: new fluorogenic probes for monoamine oxidases (MAO A and B). Optical molecular imaging of lysyl oxidase activity-detection of active fibrogenesis in human lung tissue. Modulation of lysyl oxidase-like 2 enzymatic activity by an allosteric antibody inhibitor. Development of a peroxidase-coupled fluorometric assay for lysyl oxidase.
A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes. The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to α-aminoadipic-delta-semialdehyde (allysine) by an extract from bone. A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples. Fibrosis imaging: current concepts and future directions. Molecular imaging of fibrosis: recent advances and future directions. Cellular and molecular mechanisms of fibrosis. Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer. Alterations in state of molecular aggregation of collagen induced in chick embryos by beta-aminopropionitrile (lathyrus factor). Collagen cross-linking mediated by lysyl hydroxylase 2: an enzymatic battlefield to combat fibrosis. Collagen cross-linking: insights on the evolution of metazoan extracellular matrix. Gly-X-Y tripeptide frequencies in collagen: a context for host–guest triple-helical peptides. Lysyl oxidases: from enzyme activity to extracellular matrix cross-links. Lysyl oxidase: properties, specificity, and biological roles inside and outside of the cell. Fibrosis-a common pathway to organ injury and failure. Wound repair and regeneration: mechanisms, signaling, and translation. We anticipate that our reactive collagen-based sensor will improve understanding of collagen remodeling and provide opportunities for the diagnosis of fibrotic and malignant diseases.
Change size of integral label mestrenova skin#
This synergistic probe becomes covalently anchored and lights up in vivo and in situ in response to LOX at the sites where cross-linking occurs, as demonstrated by staining of normal skin and cancer sections. Here we present a probe for the simultaneous monitoring and targeting of LOX-mediated collagen cross-linking that combines a LOX-activity sensor with a collagen peptide to chemoselectively target endogenous aldehydes generated by LOX. Visualizing and targeting this dynamic process in healthy and diseased tissue is important, but so far not feasible. During collagen maturation, lysyl oxidases (LOX) initiate the cross-linking of fibers, but abnormal LOX activity is associated with impaired tissue function as seen in fibrotic and malignant diseases. Collagens are fibrous proteins that are integral to the strength and stability of connective tissues.
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